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| Author(s): |
Maria Letícia Guindalini Melloni
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2016-01-18. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2015-12-15 |
| Advisor: | Luciana Rossini Pinto; Maximiliano Salles Scarpari |
| Abstract | |
In order to increase the knowledge of the gene network involved in sugarcane flowering induction, sugarcane cultivars were submitted to different photoperiod treatments of flowering induction and non-induction in a photoperiod facility. At 5, 10 and 20 days of induction, the +1 leaf and the leaf sheath were collected for the identification of different transcript-derived fragments (TDFs) within and between the photoperiod treatments to apply the cDNA-AFLP technique. A total of 162 TDFs were selected and re-amplified. Of these, 63 TDFs were successful in re-amplification and were cloned and sequenced. The sequences were confronted against 6 sequence databanks (SUCEST transcripts; Sorghum genome proteins; Sugarcane BACs; proteins from rice genome; Phytozome and NCBI). Similarity search was done by using the BLASTn (e-value 1e-5) tool for the SUCEST databank and sugarcane BACs while BLASTx (e-value 1e-5) was use for the other banks. Among the 63 TDFs, 23 corresponded to gene sequences while the remaining 40 represent sequences that are not deposited in these banks (no hits). The majority of the 23 sequences showed similarity with genes coding for hypothetical or predicted proteins of different organisms. Based on the protein domain analysis conducted by Pfam, six sequences may be associated with flowering induction metabolism. Among these: LM-19, LM-40 and LM-53 sequences stood out. LM-19 has similarity to the gene encoding a protein with DnaJ domain. Proteins having this domain are considered as an integrating floral signals mediator in Arabidopsis thaliana. LM-40 has similarity to the gene encoding a protein with (F-BOX) domain. This domain has a strong relationship in flowering induction processes. LM-53 has one of the predicted protein domain similar to the domain of the protein encoded by the CONSTANS gene which governs the expression of FLOWERING LOCUS T (FT), this later one encodes the florigen. Generally the cDNA-AFLP technique was effective in identifying TDFs across the flowering inductive and non-inductive photoperiodic treatments. The results suggest that LM-19, LM-40 and LM-53 sequences are linked to flowering induction metabolisms. Probably, most of the TDF here obtained may be involved in the flowering induction metabolism, although not yet been identified in the literature. (AU) | |
| FAPESP's process: | 13/24020-0 - Identification of flowering induction candidate genes in sugarcane |
| Grantee: | Maria Leticia Guindalini Melloni |
| Support Opportunities: | Scholarships in Brazil - Doctorate |