Isolation and characterization of deteriorated sectors of Abnc strain of Aspergillus nidulans
Full text
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| Author(s): |
Renata Castiglioni Pascon
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 1994-12-19 |
| Advisor: | Aline Aparecida Pizzirani Kleiner |
| Abstract | |
The filamentous fungi Aspergillus nidulans has been considerated an excellent standard organism to both, classical and molecular genetic studies, due to a great number of mutants and to its easy genetic manipulation. In this work a strain called Abnc was used. It holds a duplicated segment of chromosome I translocated to chromosome II, which leads to mitotic instability, causing 3 types of sectors to appear: improved, deteriorated and heterokariotic. Besides this duplication, the strain carries a mutation, bncA1 responsable for bi and trinucleated conidia formation. The goals of this work was to analyse deteriorated sectors coming from Abnc strain, by means of classical genetics and citology. Sexual cycle could not be conducted since a high degree of cleistotecia abortion was observed. Citological analysis using conidiogenesis and nuclear stainning with Giemsa have enable to detect morphological alterations due to bncA1 mutation and some morphological alterations caused by deteriotion. (AU) | |