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| Author(s): |
Renata Cabrera de Oliveira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2014-08-08 |
| Examining board members: |
Susanne Rath;
Ademilson Espencer Egea Soares;
Jonas Augusto Rizzato Paschoal;
Ivo Milton Raimundo Júnior;
Helena Teixeira Godoy
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| Advisor: | Sonia Claudia do Nascimento de Queiroz; Susanne Rath |
| Abstract | |
The extensive use of pesticides in agriculture crop has led to the presence of residues in food and environmental contamination, especially in countries with great agricultural potential, such as Brazil. To assess the presence of pesticide residues in the environment, honeybees and bee products have been mentioned as potential bioindicators, which can be used to monitor large areas due to long distances travelled. Therefore, the potential use of bee pollen as a bioindicator of environmental pesticides contamination has been reported in this work. For this it was necessary to develop and validate an analytical method for the determination of multiresidues in pollen, using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). For the definition of optimum extraction conditions, two procedures were evaluated: QuEChERS, and partition with acetonitrile. The QuEChERS proved to be more efficient (improved selectivity and extraction efficiency), and was validated for the determination of 27 pesticides. Sorption studies showed that pesticides are strongly sorbed in pollen. Unquantifiable levels of pesticides surveyed were found in the samples from experimental apiary at Embrapa in Jaguariúna/SP, while the presence of pesticide residues in samples provided by apiarists from Ribeirão Preto/SP was confirmed and quantified. The validated analytical methods proved to be efficient and can be used in environmental monitoring for the presence of pesticide residues. The results confirm the potential of bee pollen as a bioindicator of environmental pesticides contamination (AU) | |
| FAPESP's process: | 10/18253-4 - Evaluation of the Potencial Use of Bee Pollen as Bioindicator of Environmental Pesticide Contamination. |
| Grantee: | Renata Cabrera de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |