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| Author(s): |
Fabio Henrique Krenchinski
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2022-08-03. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu |
| Defense date: | 2022-06-10 |
| Advisor: | Caio Antonio Carbonari |
| Abstract | |
The objectives of the present work were to understand the effects on the content of lignin, phosphorus, components of the shikimic acid pathway, growth parameters and productivity, as well as through RNASeq to understand which genes and pathways are enriched by the application of subdoses of glyphosate in NR soybean, RR and RR2. In addition, the form of application of sub-doses in seed treatment was evaluated. For this, five experiments were carried out in a greenhouse in duplicate and four field experiments were carried out in different cultivation sites. In the foliar application of glyphosate in the NR cultivar, the dose range of 5.6-45 g a.e ha-1, in the RR the range of 45-720 g a.e ha-1 and RR2 the dose range of 11.25-180 g a.e ha-1, provided an increase in biomass accumulation of 12-28% in relation to the control. In the seed treatment for cultivar NR the dose range of 45-180 g a.e ha-1, for cultivar RR the doses of 90-360 g a.e ha-1 and for cultivar RR2 the range of 90-180 g a.e ha-1, provided an increase in biomass accumulation of 16-60% in relation to the control. The doses of 90 and 180 g a.e ha-1 of glyphosate in the seed treatment provided greater growth and biomass accumulation for soybean cultivars RR and RR2. We identified 587 DEG present in several enriched pathways. Lignin catabolism pathways were observed, resulting in a reduction of its contents after glyphosate application. Soybean cultivars showed higher phosphorus concentration when applied with sub doses of glyphosate, although no pathways and genes related to this process were observed. Components of the shikimic acid pathway were affected with the application of glyphosate, and appear to be linked to several enriched pathways. The application of sub-doses of glyphosate increased the content of phosphorus, amino acids, salicylic acid, growth and biomass accumulation of RR and RR2 soybean plants, in addition to reducing lignin levels. The application of AIP showed that the reduction of lignin is not the only responsible for the increase in biomass in RR and RR2 soybean plants. Regarding grain yield, in general, there were yield increases of around 4 to 38%, varying according to location and soybean varieties studied, mainly for glyphosate applied in seed treatment and foliar application + seed treatment in relation to the witness. The application of sub-doses of glyphosate can be performed in the treatment of soybean seeds. Phosphorus, lignin and shikimic acid pathway components, as well as the enriched pathways found by RNAseq, are involved in stimulating glyphosate underdoses in NR, RR and RR2 soybean cultivars. Thus, the sum of these factors is responsible for the stimulus effect in soybean. (AU) | |
| FAPESP's process: | 18/13719-7 - Glyphosate effect in gene expression, lignine and phosphuros content and yield of soybean with cp4-EPSPS gene |
| Grantee: | Fábio Henrique Krenchinski |
| Support Opportunities: | Scholarships in Brazil - Doctorate |