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| Author(s): |
Caroline Bertoncini Silva
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2022-07-01 |
| Examining board members: |
Vivian Marques Miguel Suen;
Luiz Osório Silveira Leiria;
Guilherme Vannucchi Portari;
Diane Meyre Rassi
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| Advisor: | Vivian Marques Miguel Suen; Priscila Giacomo Fassini |
| Abstract | |
Data from the World Health Organization estimates that approximately 13% of the adult world population are individuals with obesity. As a common cause of obesity, an imbalance between energy intake and expenditure, results in an excessive accumulation of body fat and low-grade chronic inflammation. Curcumin is found in the rhizome of Turmeric longa L. Several studies have demonstrated the effects of this substance in metabolic disorders, inflammation, and obesity. Thus, the aim of this study was to investigate the effect of different doses of curcumin on the gut microbiota, on body weight, on the lipid and glycid profile and on the inflammatory profile of mice fed a highfat diet. Fifty male mice were evaluated, which were divided into 5 groups of 10 animals each: standard diet (SD), high-fat diet (HFD), high-fat diet with 50 mg/kg of body weight of curcumin (HFDC50), high-fat diet with 250 mg/kg of body weight of curcumin (HFDC250) and high-fat diet with 500 mg/kg of body weight of curcumin (HFDC500). During the experimental period (12 weeks), food intake and body weight were assessed weekly. The glucose tolerance test was performed 48 hours before euthanasia. At the end of the experiment, the animals were euthanazied and blood, stool and tissue samples were collected for biochemical, histological and molecular analysis. All analysis considered a significance of 5% and were made by Kruskal-Wallis and ANOVA. When observing the percentage of weight gain, the SD group had a lower gain when compared to others and, although not different, the HFDC50 group had a lower gain (~16%) compared to the HFD group. For brown adipose tissue, greater weight was observed in the HFDC250 and HFDC50 groups had a greater weight of this tissue (43% and 29%, respectively) compared to the HFD group. When analyzing the protein expression of IL-10 in the epididymal adipose tissue, an increase was observed in the HFDC250 and HFDC500 groups when compared to the HFD group. For TNF-α gene expression in the liver and in the retroperitoneal adipose tissue, it was observed that the three doses promoted a reduction when compared to the HFD group. As for IL-10, when analyzed in the liver, there was an increase in doses of 50mg and 250mg compared to the HFD group. An increase in M2 macrophage marker gene expression (MRC-1) was also observed at 250mg and 500mg doses. Regarding the gut microbiota, at phylum level, for Actinobacteria, the HFDC250 and HFDC500 groups had an increased abundance when compared to the HFD group. For Proteobacteria, the HFDC50 group showed a decrease in abundance (~49%) when compared to the HFD group. At gender level, a reduction in Bacteroides was observed in the HFDC50 and HFDC500 groups compared to the HFD group and an increase in Clostridium cluster XIVa was observed in the HFDC50 and HFDC250 groups compared to the HFD group. In general, it can be concluded that the effects of curcumin are possibly dose-dependent, specifically, a lower dose of curcumin appears to be more effective than higher doses, as observed in the gut microbiota profile and in the inflammatory response. (AU) | |
| FAPESP's process: | 18/06094-0 - Lipid, inflammatory and intestinal microbiota profile of mice fed a high-fat diet submitted to supplementation with different doses of curcumin. |
| Grantee: | Caroline Bertoncini Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |