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| Author(s): |
André Pegoraro Poor
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2022-06-30 |
| Examining board members: |
Andrea Micke Moreno;
Roberto Maurício Carvalho Guedes;
Marcos Bryan Heinemann;
Terezinha Knöbl;
Luís Guilherme de Oliveira
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| Advisor: | Andrea Micke Moreno |
| Abstract | |
Vulvar discharges and urogenital infections cause serious economic damage to pig producers, whose most frequent clinical signs include the presence of purulent secretion in the vulvar region, reproductive failure, lack of appetite and poor body condition of the sows. However, the etiological agents involved in these infections, the susceptibility of these agents to antimicrobials, as well as the bacterial population resident in the reproductive tract of swine females are poorly studied, which makes it difficult to critically evaluate the treatments currently employed. Therefore, the objectives of this study were to isolate and identify the agents present in purulent vulvar discharges of sows by traditional bacterial culture, as well as to characterize the vaginal microbiome of sows with and without purulent vulvar discharges, through the metagenomics of the 16SrRNA gene. In addition, Escherichia coli isolates were evaluated for the presence of genes related to extraintestinal infections (ExPEC). MALDI-TOF mass spectrometry made it possible to identify a wide variety of bacteria in the reproductive canal of swine females. Escherichia coli, Streptococcus spp., Staphylococcus spp., Corynebacterium diphtheriae and Trueperella pyogenes are among the main isolated agents. Escherichia coli strains have several of the virulence factors associated with ExPEC, mainly in females with vulvar discharge, and more than 93% of them were considered multidrug resistant. The metagenomic analysis pointed out predominant genera similar to those indicated by MALDI-TOF mass spectrometry, such as Escherichia-Shigella, Streptococcus, and Staphylococcus, but also revealed other agents of pathogenic potential in higher abundance and associated in females with purulent vulvar discharge, such as Bacteroides pyogenes, Streptococcus dysgalactiae and Trueperella pyogenes. (AU) | |
| FAPESP's process: | 19/01192-7 - Characterization of bacterial pathogens causing metritis in swine using culture and metagenomics analysis |
| Grantee: | André Pegoraro Poor |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |