Full text
| |
| Author(s): |
Renata Cristina Godry
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2001-12-20 |
| Examining board members: |
Regina Ayr Florio da Cunha;
Arthur Gruber;
Rozangela Verlengia
|
| Advisor: | Regina Ayr Florio da Cunha |
| Abstract | |
The international literature is still concerning about the mycoplasma participation in human diseases mainly in what refer to the involvement of species like the Ureaplasma spp. and the Mycoplasma hominis in cases of infertility and perigestational altering. Studying the recent literature that reported the presence of less isolated mycoplasma species, Mycoplasma penetrans, Mycoplasma genitalium and Mycoplasma fermentans of HIV patients in our population, brought us the idea of this general picture study showing the less common species in cases of pregnant women, in addition to the Ureaplasma parvum, Ureaplasma urealyticum and M. hominis. We were have to trying to analyze the presence of the plasmid tetM in the isolated strains, responsible for the resistance of tetracycline observed in cases of persistent infection. Samples of the cervical material of 117 pregnant women during various pregnancy stages were evaluated. The procedure was to have the culture with identification and quantification of the isolated and PCR (Polymerase Chain Reaction), utilizing generic and specific \"primers\" for each one of the species. Toward this, techniques and procedures, which were already standardized by the international literature, were used. These analyzed data have shown the presence of all the mycoplasma species that were under research in the population of this study. The results of the clinical samples culture in solid as well as in liquid means have shown a high presence rate of 63,2% (74/117) in it. Among them, Ureaplasma spp. was present in 91 ,9% (68/74) of the patients, in which it was detected in significant concentrations; higher or equal to 104 CCU/µL in 82,4% (61/74) of the patients through the microtitration technique in U10 liquid means. It was observed 6,8% (5/74) of co-infection between Ureaplasma spp. and Mycoplasma spp. and in only 1,3% (1/74) Mycoplasma spp., all of them higher or equal to 103 CCU/µL. The infection rate obtained by the PCR in our study was of 73,5% (86/ 117) for mycoplasmas, and it has shown to be 2,3% (2/86) positive only for Mycoplasma penetrans; 7,0% (6/86) for Mycoplasma fermentans; 4,7% (4/86) for Mycoplasma hominis; 54,7% (47/86) for Ureaplasma spp. and 31,3% (27/86) for the co-infection. In these co-infections it was seen: 3,7% (1/27) for M. penetrans and Ureaplasma spp.; 14,8% (4/27) for M. genitalium and Ureaplasma spp.; 33,3% (9/27) for M. fermentans and Ureaplasma spp; 11, 1% (3/27) for M. genitalium and M. fermentans; 18,6% (5/27) for M. hominis and Ureaplasma spp.; 11,1 % (3/27) for M. fermentans, M. genitalium and Ureaplasma spp.; 3,7% (1/27) for M. genitalium, M. hominis and Ureaplasma spp. e 3,7% (1/27) for M. fermentans, M. hominis and Ureaplasma spp.. M. penetrans was detected in 3/86 (3,5%) samples, M. genitalium was found in 12,8% (11/86); M. fermentans in 25,6% (22/86) of the patients; M. hominis was positive in 12,8% (11/86) and detecting the Ureaplasma spp. through this technique has shown us its prevalence in 82,6% (71/86) of the samples. Among them, 76,0% (54/71) were identified as Ureaplasma parvum and 12,7% (9/71) as Ureaplasma urealyticum and 11,3% (8/71) of co-infection on the biotype samples.It was shown in the Ureaplasma parvum sera-type process, 7,4% (4/ 54) positive for sera-type 1; 25,9% (14/ 54) for sera-type 6; and 35,2% (19/ 54) for the sera-types 3/14. Co-infections between the seratypes were observed. The results of the subtype process concerning the Ureaplasma urealyticum have shown subtype 2, subtype 1/3 and some coinfections. The presence of the tetracycline resistance plasmid \"tetM\" was studied through the PCR in all the samples which were positive for Mycoplasma hominis and for Ureaplasma spp., and it was observed in 4/11 (36,4%) of the Mycoplasma hominis and 42/71 (59,2%) of the Ureaplasma spp. cases. The presence of the tetM is important in the evaluation of the treatment to be prescribed since it has been observed that the resistance of the mycoplasma to the tetracycline and its derivations has been more frequent. In Brazil, this was a pioneer study and we believe it can contribute for a better knowledge about the mycoplasma role in human pathologies. These data about the presence of species like the M. penetrans, M. genitalium, the high incidence of M. fermentans and the detection of M. hominis and Ureaplasma spp., as well as the co-infections, confirm the high incidence of these microorganisms in our environment. We have still known little about the actual role of the mycoplasmas infecting pregnancy and the consequences they will bring them, however we hope that through this study we will emphasize how important it is to conduct a wide research about the several species of mycoplasmas affecting our population. (AU) | |