Full text
| |
| Author(s): |
Gabriela Carneiro Sousa
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Botucatu. 2023-08-01. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2023-07-07 |
| Advisor: | Fabiana Ferreira de Souza |
| Abstract | |
Mammary tumors are the most frequent type of neoplasm in bitches, affecting about 50% of females. The canine species is considered an experimental model for breast cancer in women. In view of this, new approaches seeking biomarkers and therapeutical targets have found encouraging answers in the research of extracellular vesicles (EVs). EVs are nanoparticles secreted by several cell types, including cancer cells, which carry information from their cell of origin and are involved in the maintenance of the tumor microenvironment and metastases. This study aimed to investigate the association between serum EVs proteomics from bitches with mammary neoplasms and tumor malignancy. Thirty bitches were selected and divided into three groups, control (GC, n = 10) composed of bitches without tumors; bitches with grade I and II (GI, n = 13), and grade III (GII, n = 7) tumors. Blood was collected during surgical excision, and the serum was separated and prepared for isolation of EVs by size exclusion chromatography. Serum EVs were characterized by nanoparticle analysis, transmission electron microscopy. Then the samples were digested with trypsin for proteomics with a shotgun approach. The mean ± standard error for age (1.95 ± 0.33; 10.1 ± 0.60; 12.1 ± 0.70 years) and body weight (16.2 ± 2.5; 17.9 ± 3.6; 10.9 ± 3.4 kg). There was no difference in the concentration of EVs (5.5 x 1012 ± 4.4 x 1012; 1.3 x 1012 ± 0.66 x 1012; 1.1 x 1012 ± 0.88 x 1012 particles/mL) and EV size (136.2 ± 6.0; 129.3 ± 4.0; 125.5 ± 6.1 nm) between GC, GI, and GII, respectively. Our EVs results were not able to differentiate the GC from the GI and GII, however, are an important contribution for the characterization quantification and validation of serum EVs in bitches. Also, our proteomics results showed two important proteins from genes that are important for tumor suppression in humans’ keratin 18 and protocadherin 17 that showed differences in regulation among the groups and could be a target for future studies that intent to identify and validate biomarkers for the canine mammary neoplasia. (AU) | |
| FAPESP's process: | 21/07318-2 - Identification of biomarkers from blood serum extracellular vesicles in bitches with mammary tumors. |
| Grantee: | Gabriela Carneiro de Sousa |
| Support Opportunities: | Scholarships in Brazil - Master |