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| Author(s): |
Daniela Méria Ramos Rodrigues
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2023-10-25 |
| Examining board members: |
Eurico de Arruda Neto;
Fernando Chahud;
Edison Luiz Durigon
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| Advisor: | Eurico de Arruda Neto |
| Abstract | |
Respiratory syncytial virus (RSV) is one of the most important pathogens in child health, and by the age of 5, practically 100% of children have already been infected at least once by the agent. Studies from our research group detected RSV in asymptomatic children\'s tonsils and in lymph nodes from adult patients with benign lymphoid hyperplasia, suggesting that RSV causes prolonged and/or persistent infection. IL-10 is an important molecule in the establishment of persistent viral infections. In the present study we investigated the RSV persistence in a murine model, comparing wild type and knock out of IL-10 animals. C57BL6 wild type and knock out mice aged 3 to 5 days were infected intranasally with 104 pfu of RSV in a volume of 6.6 µL. The Lung, kidney, brain, spleen, thymus and liver were collected at 7, 15, 40, 80, 120 and 150 days post infection. We found RSV genome 150 days after infection in the liver, thymus, spleen, kidney, and brain in wild-type animals, but not in IL-10 knock out animals. In the latter, 15 days after infection no virus was detected. The viral load decreased over time, which confirms the essential role of IL-10 for persistence. In both groups, both IL-10 knock out and wild-type, infected animals showed tissue damage compatible with pneumonia and bronchiolitis. Furthermore, the altered expression of IFN γ in the acute phase in animals without IL-10 indicates the beginning of viral elimination, compatible with the decrease in genome copies found in this group. Thus, the RSV murine model becomes useful for studying mechanisms of RSV persistence and the role of IL-10 in prolonged infection. (AU) | |
| FAPESP's process: | 22/01238-0 - Respiratory Syncytial Virus Persistence in experimental model |
| Grantee: | Daniela Méria Ramos Rodrigues |
| Support Opportunities: | Scholarships in Brazil - Master |