In vitro analysis of dentin remineralization with the use of glass ionomer cement, with or without additional sources of calcium, or with calcium silicate based material

Author(s)

Handially dos Santos Vilela

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO)
• Defense date: 2023-10-10

Examining board members

Roberto Ruggiero Braga; Taís Scaramucci Forlin; Marcela Charantola Rodrigues; Tamara Kerber Tedesco

• Advisor: Roberto Ruggiero Braga

Abstract

CHAPTER II Objective: to evaluate the effect of the incorporation of hydroxyapatite (H A p) or calcium silicate ( C a S i; Biodentine, Septodont) on the compressive strength (C S , MPa) and ion release of an ionomeric cement GIC ; Fuji 9 Gold Label, GC) and verify the effect of these modified materials on demineralized dentin. Methods: GIC, GIC +5%H A p and GIC + C a S i (mass) were evaluated for C S after 24h and 7d in water and ion release (Ca 2+ and Sr 2+2+: inductively coupled plasma optical emission spectroscopy; F --: ion specific electrode) o ver 56 d. For remineralization assays, partially demineralized dentin discs were kept in contact with the materials in simulated body fluid (SBF) at 37 C by 56 d. The mineral:matrix ratio (MM R ) was determined by ATR FTIR spectroscopy. Hardness and modulus of elasticity of dentin were obtained in ultramicrodurometer. Data were analyzed by ANOVA/Tukey test ( =0.05). Results: The addition of H A p increased the S C of the GIC only after 24 h. On the other hand, the substitution of glass by C a S i reduced the C S in the two evaluated times. In general, the presence of H A p or C a S i increased ion release in relation to control (p<0.05). After 8 weeks, there was no difference in MMR or in the mechanical prope rties of dentin between groups (p>0.05). Conclusions: The substitution of glass by H A p or C a S i affected C S differently. Despite the greater release of ions, no differences were observed regarding dentin remineralization. CHAPTER III Objective: to evaluate the effect of the incorporation of calcium orthophosphates (DCPD dicalcium phosphate dihydrate or TCP tricalcium phosphate) on the degradation and release of ions of an ionomeric cement ( GIC ; Fuji 9 Gold Label, GC) and verify the effect of these ma terials and a cement based on calcium silicate (Biodentine, Septodont) on demineralized dentin. Methods: GIC , GIC +5%DCPD, GIC +15%DCPD, GIC + TCP, GIC + TCP (by weight ) and Biodentine were evaluated for degradation in deionized water after 7 d and ion release (Ca 2+ and Sr 2+2+: optical emission spectroscopy with inductively coupled plasma; F --: ion specific electrode) over 56 d. Partially demineralized dentin discs were kept in contact with materials in simulated body fluid (SBF) at 37 C by 56 d. The mineral:matrix ratio (MMR) was determined by ATR FTIR spectroscopy. Hardness and modulus of elasticity of dentin were obtained in ultramicrodurometer. Data were analyzed by ANOVA/Tukey test ( =0.05). Results: The mass loss of G IC and Biodentine were similar (p>0.001). The addition of DCPD or TCP promoted greater degradation to G IC (16.4 73.8%). The release of Ca 2+ from Biodentine and GIC ( g cm 2 ) were, respectively, 4,737.0 735.9 and 13.6 1.6. The addition of DCPD or TCP to GIC increased the release of Ca 2+ (1.5 17x), Sr 2+ (1.1 2.2x) and F (1.7 3.1x, p<0.001). Only Biodentine showed higher MMR than control after 56 d (Biodentine: 69.2 18.5; other groups: 5.6 1.2 11.8 1.5, p<0.05) and increased hardness (3.3x) and modulus of elasticity of dentin (2.4x) along the lesion (p< Conclusion: Biodentine was effective in dentin remineralization. GIC , with or without additives, did not alter the characteristics of demineralized tissue. (AU)

• FAPESP's process: 20/12761-0 - In vitro analysis of dentin remineralization using glass ionomer cement with or without calcium orthophosphate particles or with mineral trioxide aggregate
• Grantee: Handially dos Santos Vilela
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)