13C Leucine Incorporation in malnourished rats: effect of glutamine supplementation

Author(s)

Andrea Ferreira Schuwartz Tannus

• Document type: Doctoral Thesis
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC)
• Defense date: 2004-12-16

Examining board members

Julio Sérgio Marchini; Mauro Velho de Castro Faria; Alceu Afonso Jordao Junior; Isis do Carmo Kettelhut; Francine Brambate Carvalhinho Lemos

• Advisor: Julio Sérgio Marchini
• Field of knowledge: Health Sciences - Nutrition
• Indexed in: Banco de Dados Bibliográficos da USP-DEDALUS; Biblioteca Digital de Teses e Dissertações - USP
• Location: Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto

Abstract

Nutritional status recovery is thought more effective when there are amino acids, especially, glutamine supplementation. The aim of the study was to verify the effect of glutamine on duodenal mucosa protein synthesis in the nourished or malnourished rats. The experimental groups animals received oral diet glutamine supplementation (0.42g/kg/day) for 14 days while the control group did not. Thereafter, kinetic study with L-[1-13C]-leucine to 4-h and biopsies of duodenal mucosa were done. The malnourished groups showed 25% weight loss with increase urinary nitrogen. Amino acid concentration in the plasma of control group was not significantly different with from that of the experimental groups. The result showed that 13C enrichment in the plasma and biopsy sample of the malnourished group is higher than that of the nourished group, but that of the fractional synthetic rate (FSR) is inverse; FSR of the nourished group is higher 0.00440.0005/h than that of the malnourished group 0.00370.0007/h.We conclude that oral supplementation glutamine does not acutely stimulate duodenal protein incorporation in malnourished rats, regardless the refilling protocol, with or without glutamine. (AU)