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| Author(s): |
Vivian Yochiko Samoto
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. , gráficos, ilustrações, tabelas. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2006-04-10 |
| Examining board members: |
Maria Angélica Miglino;
Ana Flávia de Carvalho;
Angelo João Stopiglia
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| Advisor: | Maria Angelica Miglino |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine |
| Indexed in: | Banco de Dados Bibliográficos da USP-DEDALUS; Biblioteca Digital de Teses e Dissertações - USP |
| Location: | Universidade de São Paulo. Biblioteca da Faculdade de Medicina Veterinária e Zootecnia; FMVZ/T.1651 |
| Abstract | |
The adult stem cells have a capacity to transform in different kinds of tissue. It is emerges like a new treatment modality of cardiac diseases, so, an evaluation in cell distribution and quantification by different routes of infusion in heart are necessary, because the microenvironment and the interaction cell/cell age in the transdifferentiation and fusion process. It was used 09 healthy mongrel dogs, with 25 -30kg of weight, which was performed a punction of iliacal crest, bone marrow mononuclear cells separation by Ficoll density, stained with Hoechst and infusion by intracoronary, transendocardial and retrograde routes. The marked cells was observed in all cardiac tissue, however, the pattern of distribution of the same ones differs, however in the transendocardial and retrograde route the cells could be founded mainly into the connective tissue - epicardium and sub-epicardic connective tissue and in the intracoronary route, the cells was observed mainly in the muscular interstice. It was observed a significant number of cells in the atrial, basal, medium and apical cardiac region when related with the route of infusion. (AU) | |