| Author(s): |
Rogerio de Castilho Jacinto
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Piracicaba, SP. , ilustrações. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2007-01-23 |
| Examining board members: |
Brenda Paula Figueiredo de Almeida Gomes;
Carlos Eduardo da Silveira Bueno;
Haroun Neamath Shah;
Francisco José de Souza Filho;
Eduardo Dias de Andrade
|
| Advisor: | Brenda Paula Figueiredo de Almeida Gomes |
| Field of knowledge: | Health Sciences - Dentistry |
| Indexed in: | Base Acervus-UNICAMP; Biblioteca Digital da UNICAMP |
| Location: | Universidade Estadual de Campinas. Biblioteca Central Cesar Lattes; T/UNICAMP; J119r; Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Biblioteca Prof. Dr. Carlos Henrique Robertson Liberalli; T/UNICAMP; J119r |
| Abstract | |
The aim of this study was to analyse the microflora isolated from infected root canals of symptomatic or asymptomatic teeth, to quantify the presence of endotoxins; to correlate the presence of specific bacteria and the amount of endotoxins with endodontic symptomatology; and to investigate the antibiotic susceptibility of anaerobic bacteria isolated from infected teeth with periapical lesions against 8 antibiotics through the E-test. Microbial samples were taken from 90 root canals of teeth with necrotic dental pulp, and analysed using rigorous culture procedures. Other 50 samples were collected from infected symptomatic or asymptomatic root canals in order to be analysed by a chromogenic test for the endotoxin quantification. Statistical analysis used a Pearson x2 test or a one-sided Fisher's Exact test, as appropriate. A total of 400 cultivable isolates were recovered from 69 different microbial species and 22 different genera. Eight per cent of the bacteria were were strict anaerobes and F. nucleatum was the most frequently isolated species. Root canals from symptomatic teeth harboured more obligate anaerobes and a larger number of bacterial species than the asymptomatic teeth. Relationships were found between specific microorganisms, especially Gram-negative anaerobes and the presence of pain or history of pain, tenderness to percussion, pain to palpation and swelling. High concentrations of endotoxins were found in root canals of symptomatic teeth and there was a positive correlation between endodontic signs and symptoms and the concentration of endotoxins in infected root canals. Amoxicillin, amoxicillin + clavulanate and cephaclor were effective against all the strains tested. Our results suggested that specific bacteria and endotoxins are associated with endodontic symptoms of infected teeth and that the majority of the anaerobic species tested was susceptible to all antibiotics studied. (AU) | |