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| Author(s): |
Raquel Cristine Kuhn
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos |
| Defense date: | 2006-09-13 |
| Examining board members: |
Francisco Maugeri Filho;
Eliana Setsuko Kamimura;
Marlei Barboza Passoto;
Maria Isabel Rodrigues
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| Advisor: | Francisco Maugeri Filho |
| Abstract | |
Fructooligosaccharides (FOS) are nowadays considered natural food ingredients because of their beneficial effects in proliferating bifidobactéria in human colon; FOS are classified as prebiotics. Some recent studies show that the chromatographic separation of monosaccharides and a mixture of the disaccharides can be improved by using Y-zeolites, being promising in the separation of oligosaccharides. In this study, a column packed with zeolite was used to study the separation of oligosaccharides. The enzyme, which produces FOS from sucrose, was isolated from Rhodotorula sp., produced selectively GF2 (kestose), GF3 (nystose) and GF4 (frutofuranosyl nystose). The final yield of FOS was 52% when 50% sucrose was used as substrate. For the separation of fructooligosaccharides was used column packed with Ba2+ - exchanged Y zeolites. Efficiency of separation was used as a criterion to characterize the effectiveness of the separation. Effects of temperature (400C a 500C), amount of mixture injection (1 a 3 mL), flow rate (0,08 a 0,12 mL.min-1) and desorbent composition (ethanol 40 a 60%) were investigated, the according to a fractionary fatorial design; the flow rate was a significant factor under the response of efficiency of separation. The study of separation of sugars showed that the most favorable conditions for this separation were with utilization of two columns in series with ethanol 60% as desorbent, temperature of 500C, flow rate of 0,08mL.min-1 and amount of mixture injection of 1 mL. The values for the separation efficiency were 0,60 for oligosaccharides-glucose, 1,00 for oligosaccharides-fructose, 0,22 for oligosaccharides-sucrose, 0,43 for glucose-fructose, 0,82 for glucose-sucrose and 1,23 for fructose-sucrose (AU) | |