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| Author(s): |
Emilianne Miguel Salomão
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2010-05-03 |
| Examining board members: |
Maria Cristina Cintra Gomes Marcondes;
Miguel Arcanjo Areas;
Maria Alice Rostom de Mello;
Dora Maria Grassi Kassisse;
Claudio Alexandre Gobatto
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| Advisor: | Maria Cristina Cintra Gomes Marcondes |
| Abstract | |
The intense nutrients mobilization of host tissues, due to neoplastic growth, leads to host cachexia state. Most cancer patients have cachexia, which is characterized by involuntary weight loss through increase in protein muscle depletion and decrease in protein synthesis process. In these cases, there is reduction in quality and life expectancy. Leucine (BCAA) is used as an energy source by skeletal muscle, precursor of gluconeogenesis or acts as cell signaling and physical exercise promotes increased consumption of glucose, reducing the levels of circulating glucose and insulin, providing less supply of substrate to tumour. In this work, our main interests were to minimize the metabolic alterations in tumor-bearing host associated with nutritional supplementation of leucine and moderate aerobic exercise during Walker 256 tumour growth and whether this association could avoid or protect muscular depletion, analyzing protein metabolism through protein synthesis and catabolism process, and muscle carbohydrate metabolism through analysis of glucose metabolism, by evaluating glycolytic pathway and muscle glucose receptor in rats. Thus our results found: 1) Decrease in body weight gain after tumor implantation. 2) Decreased synthesis and increased muscle protein degradation accompanied with lower protein content and expression of myosin. 3) Accordingly, increased expression of ubiquitin-subunits proteosome. 4) Increase of pro-inflammatory cytokines. 5) Reduction of serum glucose, insulin and glucagon increase. 6) Reduction of gene expression of muscle glucose transporter, Glut-4. 7) Decrease in diameter of skeletal muscle fibers. Since dietary supplementation with leucine and / or exercise in the long run, promote the animals with tumor: 1) Improvement in body weight gain, reducing the tumor burden in some groups. 2) Decrease in skeletal muscle degradation, associated with increased expression of muscle myosin. 3) Improve the concentration of pro-inflammatory cytokines. 4) Improve the gene expression of muscle glucose transporter, Glut-4. 5) It also improves the diameter of muscle fiber (AU) | |