Full text
| |
| Author(s): |
Daniela Soares Razolli
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2011-07-26 |
| Examining board members: |
Licio Augusto Velloso;
Adriana Souza Torsoni;
Carla Roberta de Oliveira Carvalho
|
| Advisor: | Licio Augusto Velloso |
| Abstract | |
The ionotropic NMDA receptor is a glutamate receptor involved in a number of essential functions in the central nervous system. The anomalous expression or activation of this receptor can contribute to development of diseases such as Parkinson and Alzheimer. A previous study performed in our lab revealed that only two out of 1.176 genes were simultaneously modulated in the hypothalamus of rats exposed to two distinct thermogenic stimuli, i.e., exposition to low temperature and feeding a high-fat diet; the 2B NMDA receptor subunit and the GTP protein G. This fact suggests that energy expenditure is a complex and finely controlled process. In addition, previous data showed co-localization between NMDA receptor and MCH neurons, which is one of the most important hypothalamic neurotransmitters involved in the energy expenditure control. Thus, this study aimed at evaluating the expression and function of NMDA receptor in the hypothalamus of rats fed on control or high-fat diets. Since the hypothalamus is responsive to leptin and insulin, we evaluated if glutamate can modulate hypothalamic neurotransmitter expression through the leptin pathway. Rats were treated via intracerebroventricular (icv) with glutamate or glutamate followed of AG490 (a chemical inhibitor of JAK2 - inicial leptin pathway protein). Our results showed that icv glutamate reduce food intake and body weight, modulating the orexigenic and anorexigenic neurotransmitters through a mechanism, at least in part, dependent on leptin pathway (AU) | |
| FAPESP's process: | 09/11331-2 - Evaluation of different NMDA glutamatergic receptor subtypes in rats hypothalamus |
| Grantee: | Daniela Soares Razolli |
| Support Opportunities: | Scholarships in Brazil - Master |