Full text
| |
| Author(s): |
Maraisa Greggio Delboni
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2006-12-14 |
| Examining board members: |
Brenda Paula Figueiredo de Almeida Gomes;
Carlos Eduardo da Silveira Bueno;
Alexandre Augusto Záia
|
| Advisor: | Brenda Paula Figueiredo de Almeida Gomes |
| Abstract | |
The aim of this study was to identify Enterococcus spp., Staphylococcus spp., Candida spp. and enterobacteria in the saliva, to identify microorganisms in the restorative material of the crown and in the root canal of 30 failed endodontically treated teeth with periradicular lesion and to test antibiotic susceptibility of E. faecalis isolates from obtured root canal. The transport media, specific culture media and adequated gaseous requirement were used to isolate as many as strict and facultative anaerobes as possible. Seleted media were used to isolate Enterococcus spp., enterobacteria and yeasts. From the 114 microorganisms isolated from the root canais, 81.5% were facultative anaerobes and 18.5% strict anaerobes, 86% were Gram-positive and 14% Gram-negative microorganisms. The most frequently bacterial genera recovered from the root canais were: Staphylococcus (15/50%), Streptococcus (11/36.7%), Actinomyces (12/40%), Enterococcus, Gemella (8/26.7%), Propionibacterium, Clostridium (4/13.3%), Peptostreptococcus, Bifidobacterium,Eubacterium (3/10%), Lactobacillus, Prevotella (2/6.7%) and Candida (1/3,3%). Enterococcus spp., Staphylococcus spp., Candida spp. and enterobacteria were isolated in 26.7%, 50%, 3.3% and 16.7% of the canaIs, in 20%, 40%, 6.7 % e 13.3% of the crown and in 43.3%, 56.7%, 50% and 26.7% of saliva, respectively. Antibiotic sensivity of Enterococcus faecalis isolates (8/30) were tested for their susceptibility/resistance and it was accomplished with the E-testSystem. Enterococcus faecalis were 100% susceptible,to amoxicilin combined with clavulanate, gentamicin, 87.5% to amoxicilin and moxifloxa~in and 60% to ciprofloxacin. However, 100% of the E. faecalis isolates were resistant .to clindamycin and to metronidazol, 87.5% to azithromycin and rifanpicin. It was concluded that root canais with failed endodontic therapy comprise predominantly facultative anaerobic Gram-positive microorganisms. Moreover, Enterococcus spp., Staphylococcus spp., Candida spp. and enterobacteria could also be detected in the saliva and in the root canaIs. The effective antibiofics for E. faecalis were amoxicilin combined with clavulanate and gentamicin (AU) | |