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| Author(s): |
Éfani Caroline de Freitas Banzi
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2012-03-15 |
| Examining board members: |
Regina Maria Puppin Rontani;
Silvio Issao Myaki;
Elisa Maria Aparecida Giro;
Fernanda Miori Pascon;
Fabiana Scarparo Najufel
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| Advisor: | Regina Maria Puppin Rontani |
| Abstract | |
This Thesis present three chapters. The Chapter 1 evaluated the effect of bonding systems Clearfil SE Protect (primer with antibacterial monomer) (CP), Clearfil SE Bond (CB) e Adper Single Bond 2 (SB) and 2% digluconate chlorhexidine (CHX) on Streptococcus mutans. Viable bacteria were verified after that dentine slices contaminated with S. mutans were cleaned or not with CHX and treated with adhesive systems, and after that adhesive systems discs were incubated in bacterial suspension. The bacterial counts were analyzed using the Friedman test, ANOVA-R and t test (LSD), and the inhibitory zones were analyzed using the Kruskal-Wallis and t test (LSD) (p<0.05). CP when comparing with CB showed higher antibacterial effect after 60 min of bacterial contact. CHX did not improve the antibacterial effect of the bonding systems. Cured CP specimens showed an inhibitory effect on the growth of S. mutans on the agar surface. It was concluded that treatment of the dentin surface with CHX did not have an effect on S. mutans before applying bonding systems. CP exhibited considerable antibacterial activity and may be useful for reducing cariogenic bacteria. The Chapter 2 investigated the antibacterial effect of CP and CB against Streptococcus. Actinomyces and Lactobacillus. Discs of adhesive systems were kept in contact with 150 ?L of single or mixed bacterial suspension by 15, 30 and 60 minutes. The metabolic activity was determined by XTT assay and the viability bacterial by MTT and Live/Dead assay. Results were submitted to ANOVA and Scheffe's f-test (p<0.05). The CP showed bactericidal effect on the bacterial suspension compared with CB and buffer control (bacterial suspension without treatment), in all three assays. To three mixed bacterial suspensions the CP presented effect after 60 contact with bacteria. It was concluded that the CP was bactericidal after cured against cariogenic bacteria. The Chapter 3 evaluated the effect of CP, CB and CHX on the bond strength (BS) and bonding degradation of the resin/dentin interface after thermomechanical cycling (TM). The BS, bonding degradation, marginal adaptation, nanoleakage were analyzed after the specimens demineralized dentin were cleaned or not with CHX, treated with CP or CB and received or not TM (500 cycles thermal/100.000 cycles mechanical). Data were submitted to three-way ANOVA and Tukey's test (5%). The failure mode, percentage of gap marginal and the nanoleakage were evaluated descripitivelly. There was no difference significance to bond strength among group that received or not TM; however, the CHX maintained the bond strength for specimens of TM group and showed mixed failure for theses groups. Marginal gap was observed on all specimens, but to TM group the percentage of marginal gap was increased. All groups showed nanoleakage at the resin/dentin interfaces and an increase in silver deposition could be notice after thermomechanical cycling. It was concluded that adhesive system did not affect the bond strength. CHX increased the bond strength and had a positive effect to prevent nanoleakage after thermomechanical cycling (AU) | |