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| Author(s): |
Iriana Carla Junqueira Zanin
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2005-01-03 |
| Examining board members: |
Reginaldo Bruno Gonçalves;
Aldo Brugnera Junior;
Izabel Yoko Ito;
Renata de Oliveira Mattos Graner;
José Francisco Höfling
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| Advisor: | Jaime Aparecido Cury; Reginaldo Bruno Gonçalves |
| Abstract | |
Photodynamic therapy (PDT) is an alternative antibacterial therapeutic to biofilm-related diseases such as dental caries. This thesis, comprised by 3 manuscripts was designed (1) to review the literature about the use of PDT on cariogenic bacteria, (2) to validate a streptococci biofilm model and to evaluate the effect of toluidine blue O (TBO) and a red Light Emission Diode (LED) association on viability of biofilmes formed with these bacteria and, (3) to evaluate the effect of PDT using either a HeNe laser or a red LED light on the viability of S. mutans biofilms grown in a constant depth film fermentor (CDFF). In the second manuscript, Streptococcus mutans, Streptococcus sobrinus and Streptococcus sanguinis biofilms were grown on bovine enamel blocks. In the biofilms the microbiological counting and water-insoluble polysaccharide concentrations were analyzed; while mineral loss analysis were performed on enamel blocks. Also, the antimicrobial potential of TBO at 100 mg/ml associated with a LED at 85.7 Jcm-2 on single-species biofilms were evaluated by microbiological counting. In the manuscript 3, the antimicrobial effect of PDT on S. mutans biofilmes growth on CDFF were verified either using a HeNe laser or a red LED with energy density ranging from 49 to 294 Jcm-2. The effect of TFD was verified by analyzing bacterial viability and confocal scanner laser microscopy. The review of literature demonstrated the importance and shortage of studies about PDT on cariogenic biofilms. The results of manuscript 2 showed that the tested biofilm was effective to simulate high cariogenic challenger promoting enamel demineralization. Also, significant reductions (p = 0.05) in bacteria viability were founded after PDT. At the third study, significant reductions in S. mutans biofilms counting was observed after PDT using either a HeNe laser or a LED light the both light (p = 0.05), however, the effect was restricted to external biofilm layers. In conclusion, the results of these studies showed that either a HeNe laser or a LED light in the presence of TBO showed antimicrobial effect on streptococci biofilms (AU) | |