Full text
| |
| Author(s): |
Juliana de Almeida
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2008-07-18 |
| Examining board members: |
Dora Maria Grassi Kassisse;
Eliana Cotta de Faria;
Carlos Alberto Silva;
Fernanda Ramos Gadelha;
Lila Missae Oyama
|
| Advisor: | Dora Maria Grassi Kassisse; Regina Celia Spadari Bratfisch |
| Abstract | |
There are few animal models to study the peripheral glucose uptake alterations induced by stress. Rat foot-shock stress model induces metabolic alterations similar to diabetes type 2. Considering that rat foot-shock stress model causes physiological alterations which are possible to be studied carrying out experiments in vivo or in vitro we claim in this work evaluate glucose uptake in stressed rats throughout ivGTT, clamp euglycemic-hyperinsulemic, glucose uptake and lactate production in isolated adipocytes. Our results show that: glucose uptake by isolated stressed rat adipocytes is decreased by insulin and increased by a1-AR agonists; besides ß2-AR stimulation significantly inhibit glucose uptake induced by a1-AR; there is no alteration in lactate production by isolated adipocytes rats; a1-ARs are involved in glucose uptake demonstrated in ivGTT assays; sensitivity to insulin receptors were decreased in some rats when available by clamp assays. All these results together suggested that stress induced by foot-shock in rats induces alterations in glucose uptake, mainly throughout adrenergic mechanisms, and this model could be very useful as a tool to study metabolic alterations during stress response (AU) | |