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| Author(s): |
Luciana Keiko Hatamoto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2004-07-08 |
| Examining board members: |
Renato Campanarut Barnabe;
Carmen Neusa Martins Cortada;
Marcelo Alcindo de Barros Vaz Guimarães;
Maria Denise Lopes;
Fabiana Ferreira de Souza
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| Advisor: | Renato Campanarut Barnabe |
| Abstract | |
The present study aimed to evaluate if the stress in dogs may have deleterious effects on the sperm quality and on the composition of the seminal plasma. IN addition, the effect of supplementation with vitamin E on stressed dogs was also evaluated. Eighteen male adult rotweiller dogs were randomly allocated in a 2X2 factorial treatment design (stress and no stress X vitamin E supplementation and no supplementation). Animals of the supplemented group received 500 mg of -tocopherol/animal/day. The stress was induced 7 days after the beginning of the vitamin supplementation using dexamethasone (0.01 mg/kg intramuscular for 7 consecutive days). Stress was characterized through the measurement of plasmatic cortisol, food intake, body score and weight. Semen collections were performed twice a week through digital manipulation and blood collections were performed weekly. Semen parameters used to evaluate the effect of the treatments were: ejaculate volume, density and pH, sperm motility, vigor, concentration and morphology, sperm membrane integrity (hypo-osmotic swelling test - HOST, fluorescent probe IP/DCF, triple stain, simple acrosome stain and sperm viability), total protein concentration, lipid peroxidation rate (TBARS), enzymatic activity of the antioxidants catalase, superoxide dismutase, glutatione peroxidase in the seminal plasma and plasmatic testosterone concentration. The stress influenced the ejaculate volume and pH, TBARS and percentage of dead cells with reacted acrosome (class 4 of the simple stain). Vitamin E had na effect on the percentage of sperm major defects, TBARS, percentage of cells with intact acrosome and sperm viability. The effect of vitamin E and stress was observed on sperm motility, vigor and concentration, percentage of major sperm defects, percentage of intact membrane cells evaluated through the HOST and through the fluorescent probe IP/DFC. Results obtained indicated that the stress has a deleterious effect on the spermatic cell and that the composition of the seminal plasma and the oral vitamin E supplementation may modify this effects. (AU) | |