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| Author(s): |
José Sergio de Arruda Gonçalves
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2007-08-17 |
| Examining board members: |
José Antonio Visintin;
Silvia Edelweiss Crusco;
Camila Infantosi Vannucchi
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| Advisor: | José Antonio Visintin |
| Abstract | |
The objective of the present study was to analyze the gene expression of estrogen (ERα and ERβ) and progesterone (PR) receptors in oocytes and cumulus cells on different phases of the estrous cycle in dogs. Ovaries from selected bitches were recovered after ovary-hysterectomy and sliced at low temperatures in PBS supplemented with 10% FCS. Immediately after surgery, blood samples were harvested for measurement of estrogen and progesterone serum concentration. Bitches were grouped within different phases of the estrous cycles, according to previous evaluation of colpocytology, colposcopy and serum progesterone levels. Oocytes recovered after slicing were mechanically denuded. Isolated oocytes and cumulus cells were cryopreserved and submitted to RT-PCR followed by a real time PCR for relative quantification of gene expression. Gene expression of ERα, ERβ and PR in oocytes and cumulus cells was not different among phases of the estrous cycles. For the first time it was possible to demonstrate ERα and ERβ in dog oocytes. (AU) | |