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Evaluation of salivary progesterone in bitches during periovulatory period

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Author(s):
Patricia Rotta Lopes
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
Defense date:
Examining board members:
Claudio Alvarenga de Oliveira; Silvia Edelweiss Crusco; Camila Infantosi Vannucchi
Advisor: Claudio Alvarenga de Oliveira
Abstract

Several authors have already emphasized the importance of monitoring estrous cycle in bitches and mentioned examples of how it can be done. The aim of this study was to evaluate the salivary progesterone quantification technique in order to monitor the estrous cycle in this species. To compound the experimental group, 13 bitches were used. Blood and saliva samples were collected simultaneously in all animals, starting about the first day of proestrus signs. Salivary samples were collected with a specific device: Salivette®. This method was effective, since it was possible to obtain enough volume in almost all samples to quantify progesterone. Serum progesterone was quantified by radioimmunoassay and salivary progesterone by enzyme immunoassay. Although there is an increasing, linear and positive correlation between salivary and serum progesterone (r=0,704; p<0,0001), it is not possible to use the salivary parameter to set the moment of ovulation. (AU)

FAPESP's process: 09/12528-4 - Evaluation of salivary progesterone compared to plasmatic progesterone in bitches during the estrous cycle aiming assisted reproduction.
Grantee:Patricia Rotta Lopes
Support Opportunities: Scholarships in Brazil - Master