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| Author(s): |
Daniele dos Santos Martins
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2006-12-20 |
| Examining board members: |
Maria Angélica Miglino;
Joaquim Mansano Garcia;
Irina Kerkis;
Tatiana Carlesso dos Santos;
Camila Infantosi Vannucchi
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| Advisor: | Maria Angélica Miglino |
| Abstract | |
The canine domesticity generated simultaneously in different parts of the World, that created, the begin of selection of many breeds. Since that time, dogs become to be used for different works, since follow men, that usufructed their convinience discovering most of the time that both are soffering the same ill. Dogs from the Golden Retriever breed presented a degenerative and genetically disease, homologous to Duchenne Muscular Dystrophy (DMD) in human, the dystrophin gene affection; a muscle citosckeletical protein. A possibility way to supply a correct gene shape for the affected muscle could be from the stem cell therapy from embrionic stem cell, germ cells, umbilical cord blood cells, bone marrow and perific blood. Researches with animal models are showing sucess on the treatment with fetal stem cells or pluripotents ones in different types of cronic illness, then, we aimed a treatment of the Golden Retriever Muscular Dystrophy using embrionic germ cells meantime characterization and their use on cell therapy. Were uses 16 females crossbreed (SRD), bitches were selected by the vaginal cytology exams; females were artificially inseminated and after 30 days of pregancy, the animals were histectomized. Embryos were collected to stabilish embrionic germ cells, canine fibroblasts, muscle cells, which were analysed by flow cytometre, co culture and OCT-4 detection. The results showed the paramesonephic region of embryos with 22 days of pregnancy presents undifferentiate cells(germ cells), what differs from embryos with 24 days, which primitive kidneys presented differentianted types of cells. Under culture conditions, these cells formed compact colonies with high proliferative potential, but without capacity of maintenance after 30 days. (AU) | |