Full text
| |
| Author(s): |
Aline Gonçalves
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2012-02-10 |
| Examining board members: |
Edson Aparecido Liberti;
Renato Paulo Chopard;
Flavia de Oliveira
|
| Advisor: | Edson Aparecido Liberti |
| Abstract | |
Nutritional and metabolic factors can cause serious injury of the development of dental tissue, especially when occurred in critical periods. Several studies reveal that conditions derived from early and later under nutrition interfere in insulin and IGF system activities; in these studies has been demonstrated the possible involvement of some pathologies most of them pointing to permanent in high degree (if not immediate) prospective and \"possible dangerous\" morphological and functional performance of tissues. Thus, the present study aimed to evaluate the effects of the pre and post-natal protein under nutrition, and, post-natal refeeding on the development of the dental pulp and periodontal junctional ephitelium of wistar rats, in order to find possible correlation between the metabolic and morph-functional changes arising from protein under nutrition, previously associated with studies related to the effects of these hormones. For this purpose, heterogenic animal groups were formed (n=3) divided in accordance of their diets (protein or hypo protein) and their ages into the following experimental groups: nourished (N) under nourished (S) aged 60 days (final of pubescent l periods) and renourish (R), recovered from the 22nd to 60th day old. After euthanasia, the specimens were analyzed by light microscopy (stained with Azo-carmine, Hematoxylin-Eosin and Picro Sirius, the later for collagen component analysis) and immunohistochemistry examination for identification of IGF-I expression, Insulin and respective receptors. The dental pulp turned out to be impaired in the context of undernutrition. This phenomenom was seen through disorganization of the odontoblastic layer and the stagnation of collagen components in undernourished animals. The refeeding procedure was unable to promote the recovery from any of the two parameters. With the immunohistochemistry on the dental pulp, it was found that the number of IGF-I expression was higher in group N and that there were significant differences involving these animals and those from groups S and R. Similarly, the highest expression of their receptors (IGF-IR) was found in group N, demonstrating statistical difference between this one and the group of undernourished animals. The highest average percentage of the expressions of insulin occurred in nourished animals (N), but no statistically significant difference was detected among the groups. In regard to the insulin receptor (IR), statistically significant differences were found when the group N was compared to groups S and R. In the junctional ephitelium, the undernutrition determined changes in the pattern of epithelial cells in undernourished animals and the prevalence of type III collagen fibers in the connective tissue that supports it, featuring a delay in the development of these tissues. The refeeding was not able to satisfactorily recover their structural components. About immunohistochemical expressions of all antibodies used for IGF-I, IGF-IR, I e IR in the junctional ephitelium, the statistical analysis showed no significant differences among the values found in the groups N, S and R. (AU) | |
| FAPESP's process: | 10/04845-7 - Morfoquantitave evaluation of the expressions of IGF-I, insulin and their receptors on dental pulp and junctional epithelium of the pubescent wistar rats after pre and postnatal protein undernutrition and postnatal refeeding |
| Grantee: | Aline Gonçalves |
| Support Opportunities: | Scholarships in Brazil - Master |