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| Author(s): |
Mirela Aline Real de Lima
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2009-04-14 |
| Examining board members: |
Maria Lúcia Zaidan Dagli;
Idércio Luiz Sinhorini;
Yatiyo Yassuda
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| Advisor: | Maria Lúcia Zaidan Dagli |
| Abstract | |
Nowadays there are many techniques applyed in cytogenetics in order to identify or avoid potentially hereditary genetic imbalance. Some of the techniques are used only to diagnose human diseases, but it would be important to apply them also to domestic animals, with the purpose of associating a genetic cause with the appearance of some diseases like, for example, mastocytomas. The mastocytomas are neoplasics cutaneous formation originated from mastocitos and frequently appears in dogs. Some breeds show higher susceptibity to the appearance of mastocytoma, and the boxer breed has shown the highest incidence. The objective of this study is to comparatively evaluate the boxer breed chariotype and search for a possible correlation with the mastocytoma development. There has been utilized cytogenetics bandamentos C,G e Ron as well as conventional (Giemsa) coloring analysis. For those analysis there has been utilized cultivated cells from peripheral lynphocites and boxer´s mastocytomas. The material as been analyzed and photographed in a Zeiss immersion microscope, using a 100x objective with green filter, equipped with Ikaros cytogenetic analysis software. It has been considered numerical and structural alterations. There has been analyzed 828 mithotics metaphases from peripheral lynphocites and mastocytomas cells. The analysis of metaphases from the culture of peripheral lynphocites cells did not show structural alterations and the numerical analysis has shown only one metaphase 2n=77, among 476 analyzed metaphases. In the tumoral metaphase cells analysis, among all colorations, it has been observed that 70,9% presented 2n=78, 17,4% shown 2n=77 and 9,8% 2n=76. Five metaphases (1,3%) presented 2n=79 and two cell presented 2n=75. All these numerical alterations are related with structural alterations from the centric fusion type. The obtained results shows that due to the uncontrolled growth, the tumoral cells promote rearrangements in an attempt to control a cellular division. Yet, the improvement of diagnosis technique in cytogenetics promotes the knowledge of canine mastocytoma tumoral biology, and consequently allows the new therapeutic strategy development. (AU) | |