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| Author(s): |
Thaís Sebastiana Porfida Ferreira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2007-01-30 |
| Examining board members: |
Andrea Micke Moreno;
Franco Ferraro Calderaro;
Antônio José Piantino Ferreira
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| Advisor: | Andrea Micke Moreno |
| Abstract | |
Clostridium perfringens is an important enteric disease pathogen of domestic animals and foodborne diseases of human beings. Although infections caused by C. Perfringens type C and A in swine are well studied, just few reports describe genetic relationship among strains in the epidemiological chain of Swine Clostridioses, as well as the transmission of the microorganism by the nursing female its elimination and maintenance at slaughterhouses. The aim of the present study was the isolation and genotypic characterization by Pulsed-Field gel eletrophoresis (PFGE), of C. Perfringens strains from feces and carcasses from at slaughterhouse pigs, feces from sows and their piglets, and samples of meat and bone meal. The microorganism isolation frequencies in carcasses, finishing pig feces, and meat and bone meal were 44.2%, 52.5%, 32.2%, respectively. According to Polymerase Chain Reaction (PCR) assay, only the alfa and beta 2 toxins were detected; whereas beta 2 toxin was detected barely in cases of enteritis. By means of the PFGE assay techinique the samples were characterized in 97 genetic profiles with a high discriminatory level. Strains from carcasses samples presented high similarity to strains from fecal origin. strains from meat and bone meal samples showed similar profiles to strains from sow feces samples; of sows, assimptomatic piglets and piglets with enteritis. And strains isolated from piglets (assimptomatic and with enteritis) presented low similarity to strains from sow feces samples. (AU) | |