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| Author(s): |
Simone Silveira da Costa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2003-10-29 |
| Examining board members: |
Nilson Roberti Benites;
Hélio Langoni;
Priscilla Anne Melville
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| Advisor: | Nilson Roberti Benites |
| Abstract | |
Mastitis is a complex pathologic condition which may have different etiology and consequences. Mastitis usually starts with the penetration of microorganisms through the teat canal towards mammary parenchyma and secretory cells, and an inflammatory response occurs due to the products of the microbial growth. The aim of this work was to evaluate the microbial status of milk samples collected from lactating cows with mastitis and comparing the colonies forming units (CFU)/mL and the somatic cell counts (SCC)/mL, taking into consideration the differentiation of polymorphonuclear (PMN/mL) and mononuclear (MN/mL) cells. From primiparous and pluriparous dairy cows, 506 milk samples were collected from 15 dairy farms located in the State of São Paulo, Southeastern Brazil. Samples were submitted to strip cup test and California Mastitis Test (CMT) and evaluated for CFU and SCC procedures. For microbial examinations, 112 (22.13%) samples were found negative and from the other 394, the following microorganisms were isolated: 94 (18.58%) Corynebacterium spp., 104 (20.55%) Staphylococcus spp., 73 (14.42%) Streptococcus spp., 36 (7.11%) Prototheca zopfii, 8 (1.60%) Candida spp., 2 (0.39%) Nocardia spp., and 77 (15.21%) associations among distinct microorganisms. Significant correlations were found between the CFU/mL and SCC/mL, and also with the PMN and MN cell countings. With the increased CFU/mL countings, it was observed the increase in SCC, PMN, and MN countings. The CFU/mL counts in samples with Streptococcus spp were statistically higher (P < 0.05) than the ones positive for Staphylococcus spp. and Corynebacterium spp. In case of treatments of mastitis, target must be primarily oriented to treat the mammarial glands showing clinical or subclinical mastitis with Streptococcus spp. showing high CFU/mL, and at the second stage, in case of treatment or drying the cows, therapy must be directed against coagulase-positive Staphylococcus, coagulase-negative Staphylococcus and Corynebacterium spp. (AU) | |