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| Author(s): |
Debora Maria Sansini Freitas
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2007-07-20 |
| Examining board members: |
Jorge Alberto Marques Rezende;
Ricardo Gioria;
Hugo Kuniyuki
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| Advisor: | Jorge Alberto Marques Rezende |
| Abstract | |
Papaya ringspot virus - type W (PRSV-W) and Zucchini yellow mosaic virus (ZYMV) are two potyviruses associated with severe yield losses on cucurbit crops in the State of São Paulo and other parts of the Brazilian territory. Preimmunization with mild strains has proved to be a good alternative for the control of both viruses in susceptible cultivars. The main purpose of this work was to evaluate the competition for infectable sites as a possible mechanism of cross protection between the mild strain PRSV-W-1 and the severe strain PRSV-W-C in zucchini squash (Cucurbita pepo cv. Caserta). Protective inoculation with the mild strain was done at the cotyledon and the challenge inoculation with the severe strain was applied on the first true expanded leaf, and viceversa. Different plants were challenged at three, six and nine days, respectively. No challenge protected plants and healthy plants infected with the severe strain were used as controls. Evaluations were based on the expression of the symptoms at 30 days after challenge inoculation. Attempts to recover the challenge strain from challenge inoculated and new developed leaves were also done at eight days after challenge inoculation. RT-PCR with specific pairs of primers was also used to detect both stains in some of these samples. Regardless the leaf on which the protective strain was applied (cotyledon or first true expanded leaf) there appear to be some infectable sites available for superinfection with the severe strain. When the challenge inoculation was done at three days after preimmunization, the severe strain was able to superinfect some plants, move systemically and overcome the mild strain, since these plants expressed severe symptoms. All plants become protected against the expression of the symptoms induced by the severe strain when the challenge inoculation was done at six and nine days after preimmunization. However, the severe strain was still detected in the inoculated and upper leaves of few test-plants, eight days after challenge inoculation. In addition to this, it was also determined the period of time necessary to protect zucchini squash plants with a mild strain of ZYMV, named ZYMV-M, alone and in mixture with mild strain PRSV-W-1. The results indicated that single and double preimmunized plants were protected against infection and/or expression of the homologous severe strain seven days after protective inoculation. (AU) | |