Full text
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| Author(s): |
Luciane Santini
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2010-01-27 |
| Examining board members: |
Maria Lucia Carneiro Vieira;
Rosana Pereira Vianello Brondani;
Antonio Augusto Franco Garcia
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| Advisor: | Maria Lucia Carneiro Vieira |
| Abstract | |
In the presenty study, the NBS-profiling method was used for the development of RGA (Resistance Gene Analogs) markers in two populations of Phaseolus vulgaris, one derived from a cross between \'Bat 93\' and \'Jalo EEP558\' (BJ) and the other derived from a cross between \'Carioca\' and \'Flor de Mayo (CFM). After their identification, 32 RGA markers were mapped on the BJ population and 40 on the CFM population. Nine of the markers assigned to the linkage map of the BJ population were located in the proximity to clusters of resistance already identified by other researchers. We carried out the sequencing of 32 out of the RGA detected, being 16 from each population. Five sequences derived from the BJ population and three sequences from the CFM population showed similarity to resistance proteins identified in P. vulgaris, Glycine max and Medicago truncaluta. The linkage map here generated for the CFM population was used for the positioning of QTL (Quantitative Trait Loci) for resistance to angular leaf spot (Pseudocercospora griseola (Sacc.) Crous & Braun) and powdery mildew (Erysiphe polygoni DC.). Twelve QTL were mapped, five associated to the response to the angular leaf spot and seven to the powdery mildew. (AU) | |