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| Author(s): |
Nádia Juliana Beraldo Goulart Borges
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2008-02-27 |
| Examining board members: |
Julio Sérgio Marchini;
Renato Ferreira da Silva;
Helio Vannucchi
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| Advisor: | Julio Sérgio Marchini |
| Abstract | |
Non-alcoholic fatty liver disease (NAFLD) is a common clinical pathological condition characterized by fat accumulation in the the hepatic parenchyma hepatocyte. Liver steatosis (HS) is one of the components of NAFLD and is characterized by the presence of lipids vacuoles, mainly triacylglycerol, within the hepatocites. Alterations in fat oxidation in the liver or very low density proteins lipoproteins tranport from the organ are the main etiopatogenic mechanisms involved in HS. NAFLD patogeny is multifactor, thus several factors have been associated the propensity to develop it. Presently, many drug treatments for NAFLD are being suggested, however, there have been no studies that prove their efficacy so far. The aim of this study was to assess the effects of choline and fructooligosaccharide (FOS) suplementation in Wistar rats with HS induced by high glicid diet. Forty six adult male Wistar rats weighing between 250g and 320g from the USP (Ribeirão Preto-USP) central vivarium were used. They were divided randomly into different study groups from I to IV depending on whether steatosis would be induced or not. Phase I of the study was the period corresponding to steatosis induction and phase II was when the animals received nutrient suplementation (Groups III and IV) or when they received standard diet (Group II). Group I animals (control) received the usual vivarium food, which was the same for all of them. A certain amount of that same food was kept aside for the duration of the experiment. The following variables were analyzed: weekly food intake, weight gain, urine ammonia, urine nitrogen, total cholesterol and serum triacylglycerol, liver and heart humid weight, nitrogen and tissue fat, vitamin E, malondialdehyde (MDA) and glutathione content in the liver tissue and hitopathological analysis. As observed, neither of the nutrients (choline and FOS) was efficient in reducing the amount of fat in the liver. Neither of the nutrients added was able to protec the liver from free radicals, once the MDA, a indirect marker for oxidative stress generation, showed high levels even during the treatment phase. There was a reduction in triacylglycerol levels in all steatosis induced groups, from the beginning to the end of the experiment. Fructooligosaccharide was able to reduce the levels of serum cholesterol, in relation to its basal levels, when suplemented after steatosis induction. (AU) | |