Full text
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| Author(s): |
Tiago Henrique Martins
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Carlos. |
| Institution: | Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) |
| Defense date: | 2007-04-13 |
| Examining board members: |
Maria Bernadete Amancio Varesche Silva;
Eugenio Foresti;
Giovana Tommaso
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| Advisor: | Maria Bernadete Amancio Varesche Silva |
| Abstract | |
This research aimed to enrich and to purify, in chemostats, microbial trusts capable to realize ammonium oxidation to dinitrogen (\'N IND.2\') under anaerobic conditions, using as inoculum: (Q1) biomass from nitrifying-denitrifying reactor of wastewater treatment plant of amino-acids industry (Ajinomoto) and (Q2) granular sludge from upflow anaerobic sludge blanket UASB reactor treating poultry wastes - Avícola DACAR, TIETÊ. The inoculum was enriched in chemostats with affluent flow of 18 ml/h of specific basal media with mean concentrations of 80 mg \'N-NH IND.4\'POT.+\'/L, 75.2 mg \'NO IND.2\'POT.-\'/L and 1000 mg/l of bicarbonate as sole carbon source. In Q1 and Q2 chemostats the mean efficiency of removal were 51.6% and 39.3% of ammonium, and 60.5% and 53.2% of nitrite, respectively, after 296 days of operation. In Q1 and Q2 chemostats, the mean efficiency of total nitrogen removal (\'N-NH IND.4\'POT.+\' + \'NO IND.2\'POT.-\') were 57.1% and 43%, respectively, after 296 days of operation. The purification of enriched microbial trusts was carried following methodology of density gradient by centrifugation (Percoll). The microbial trusts were observed by microscopic analysis and the diversity was evaluated by DGGE, before and after the purification by the Percoll protocol. The DGGE analysis showed changes in microbial trusts structure in Q1 and Q2 in chemostats operation period. The fluorescence in situ hibridization technique (FISH) with Amx-368 probe confirmed the presence of anammox microrganisms in both microbial trusts. The sequencing of recovered bands of DGGE was carried through ribossomal DNA 16S using universal primers for bacteria Domain that related two bands with green nosulphur bacterium using maximum likelyhood tree. (AU) | |