Analisys of PROX1 overexpression effects in an oral squamous cell carcinoma cell line

Author(s)

Maria Fernanda Setúbal Destro Rodrigues

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO)
• Defense date: 2011-12-15

Examining board members

Fabio Daumas Nunes; Ricardo Della Coletta; Pedro Michaluart Junior; Patrícia Severino; Eloiza Helena Tajara da Silva

• Advisor: Fabio Daumas Nunes; Patrícia Severino

Abstract

Homeobox genes encode transcription factors with an important role during normal development by controlling cellular proliferation and differentiation. Altered expression of PROX1 homeobox gene is related to many cancers, including those of the breast, esophagus, liver, billiary system and lymphomas. The aim of this study was evaluate the effects of PROX1 overexpression, in an oral squamous cell carcinoma cell line, on cellular proliferation and differentiation, apoptosis as well as gene expression prolfile. After overexpression of PROX1 gene in SCC9 cell line, proliferation was assessed by proliferation curve, flow citometry, BrdU incorporation to DNA and Ki67 expression. Cell differentiation was verified by immunocytochemistry to cytokeratins 1, 10, 13, 14, 16, 18 and 19 and apoptosis was measured by annexin V positive cells. Gene expression profile was analyzed by microarray in PROX1-overexpressing cells and control. PROX1-overexpressing cells showed a statistically significant decrease in proliferation as well cytokeratin 1, 13, 18 and 19 expression. No significant differences from controls and PROX1- overexpressing cells were observed in apoptosis. Microarray analyses showed differential expression of genes related to development, cellular adhesion an invasion. Our results strongly suggest that overexpression of PROX1 inhibit cell proliferation and contributes to differentiation of oral squamous cell carcinoma. (AU)

• FAPESP's process: 08/06362-3 - Microarray expression profile of oral squamous cell carcinoma cell lines overexpressing HOXD11.
• Grantee: Maria Fernanda Setúbal Destro Rodrigues
• Support Opportunities: Scholarships in Brazil - Doctorate