INFLUENCE OF ABSORBABLE DEMINERALIZED BOVINE CORTICAL MEMBRANE ON THE REPARATION PROCESS AFTER PULPOTOMY IN DOGS: MICROSCOPIC AND RADIOGRAPHIC ANALYSIS.

Author(s)

Giovana Calicchio Canova

• Document type: Master's Dissertation
• Press: Bauru.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB)
• Defense date: 2003-05-30

Examining board members

Jose Mauro Granjeiro; Clovis Monteiro Bramante; Eloi Dezan Junior

• Advisor: Jose Mauro Granjeiro

Abstract

The success of the pulpotomy is directly related to maintaining vitality and function of the pulp-dentin complex after removal of the coronary portion of the pulp and protection of the radicular pulp. Several materials have been studied for such purpose, however none has yet presented all of the physical, chemical and biologic properties desired, thereby indicating the need for a continuous search for alternative materials. Aiming the analysis of new material for the vital pulp, the purposes of this study were: i) to microscopically evaluate the behavior of dog dental pulp after pulpotomy followed by capping with absorbable demineralized bovine cortical membrane (Group I), with the hypothesis that this biomaterial could induce formation of mineralized tissue barrier after pulpotomy in dogs’ teeth, or with calcium hydroxide (Group II, control); and ii) to compare digital and conventional radiographic methods in their ability to identify the pulp responses to treatments, with the hypothesis that digital (Digora) radiographic method in comparison with conventional (periapical film) allows better identification of the mineralized tissue barrier formed after pulpotomy. Methods followed the “International Organization for Standardization" (ISO) specification #7405:1997. One dog was used for each experimental period of 7 and 70 days. Ten teeth per animal were treated according to Groups I and II. The animals were killed after the experimental periods and the teeth removed and prepared for microscopic and radiographic analysis. After the 70-day period, microscopic evaluation showed formation of a fibrous connective tissue in teeth from group I and mineralized tissue in teeth from group II. Radiographic evaluation of 32 roots demonstrated a suggestive image of mineralized tissue formation in 4 roots (all from the period of 7 days) by the digital method and 5 roots (only one from the period of 70 days) by the conventional method, all from the calcium hydroxide group. Nevertheless it was possible to conclude that: i) Although absorbable demineralized bovine cortical membrane is regarded a biocompatible material, it did not induce formation of mineralized tissue barrier after pulpotomy in dogs’ teeth; ii) the conventional radiographic method is more efficient than the digital method to identify mineralized tissue barrier, and iii) there was no correspondence between microscopic and radiographic findings after pulpotomy. (AU)