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| Author(s): |
Paulo Roberto Maciel Filho
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) |
| Defense date: | 2010-09-23 |
| Examining board members: |
Debora Rejane Fior Chadi;
Thiago dos Santos Moreira;
Maria Aparecida Visconti
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| Advisor: | Debora Rejane Fior Chadi |
| Abstract | |
Essential hypertension is a disease that affects nearly 20% of the adult population, reaching 50% of the elder, and the mechanisms of its genesis are not yet completely know. This work aim to investigate two genes related to the renal mechanisms of blood pressure in three areas of central nervous system (dorsal bulb, ventrolateral bulb and hypothalamus) by means of real time PCR, in order to valuate their possible involvement in the central mechanisms of blood pressure control. One of them is the SAH gene, whose role remains to be clarified. It has higher transcription rates in hypertensive rats (SHR) than in the correspondent normotensives WKY. The other gene codifies the angiotensin II type 1 receptor associated protein (ATRAP) inducing its internalization. Thus, besides the transcriptional differences of the ATRAP protein between SHR and WKY rats, the ATRAP codifying gene is linked to blood pressure control played by means rennin-angiotensin system through AT1 receptors. Our results provide evidence for the first time, on the expression of both genes in areas of the nervous system involved with the central blood pressure regulation. It was not possible to establish a relationship between the SAH gene expression and the development of hypertension in the SHR rat. The expression of the ATRAP codifying gene was increased in the SHR between 1 and 2 months of age, which is an important period for hypertension development in this strain. Thus, this study shows the expression of SAH gene and ATRAP codifying gene in areas of nervous system of SHR and WKY important for the central regulation of blood pressure. (AU) | |