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| Author(s): |
Thompson Eusebio Pavan Torres
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-08-18 |
| Examining board members: |
Claudimara Ferini Pacicco Lotfi;
Maria Candida Barisson Villares Fragoso;
Patricia Gama
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| Advisor: | Claudimara Ferini Pacicco Lotfi |
| Abstract | |
Different experimental models have been used to study the trophic action of ACTH in adrenal, which seems to be important in proliferation and cell death in vivo. This hypothesis was tested using hypophysectomized or dexamethasone treated rats, and through IHC we evaluate: 1-the BrdU incorporation in different adrenal cortex zone after i.p. of ACTH, FGF2 or N-POMC, 2-if the mitogenic response occurs through the MC2R, using for it an antagonist of ACTH, 3-the number of adrenocortical cells which entered into apoptosis, and if ACTH recover this number. The results show that: 1-ACTH is mitogenic in three zone of adrenal cortex; 2-The mitogenic response of ACTH occurs through MC2R; 3-Mitogenic action of FGF2 occurs in the inner zones of adrenal cortex; 4- The N-terminal POMC peptide induces proliferation in the zone glomerulosa; 5- ACTH prevents apoptosis in the adrenal cortex resulting from the surgery, 6- The alteration of cyclin E and CDKI p27 could be a likely molecular mechanism of the effects induced by ACTH. (AU) | |