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| Author(s): |
Fernanda dos Santos Almeida
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2007-06-27 |
| Examining board members: |
Mario Julio Avila Campos;
Nilson Roberti Benites;
Antonio Fernando Pestana de Castro;
Clovis Wesley Oliveira de Souza;
Jorge Timenetsky
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| Advisor: | Mario Julio Avila Campos |
| Abstract | |
In this study the bacteria of Bacteroides fragilis group was evaluated in 108 fecal samples of calves with and without diarrhea, besides virulence factors and the genetic similarity among the B. fragilis strains. Hemolysin was observed in 36.3% and in 83.7% of calves with and without diarrhea, respectively. Only 7.4% of the isolates showed hemagglutinability. Of all the isolates, the major part resisted to the action of the serum and 100% were sensitive to the imipenem and metronidazole. There was resistance to the used heavy metals. Plasmids were detected in 7.4% isolates. Among 58.8% ß- lactamase producing, 19.7% and 26.0% strains of calves with and without diarrhea, respectively the cepA gene was detected, that was also observed in 5.5 kb plasmids. The cfiA gene was observed in 16.5% of the diarrheic isolates and 12.6% of non-diarrheic, but not in plasmids. The nanH gene was detected in 21.8% of the isolates and the bft gene only in two diarrheic isolates. The genetic similarity among the B. fragilis showed the heterogeneity of the bacteria. (AU) | |