Pesquisa de vírus entéricos humanos em lodos de esgoto originários de duas ETEs do Estado de São Paulo: estabelecimento e avaliação de metodologia para recuperação e detecção viral.

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Author(s):	Karina Medici Barrella Total Authors: 1
Document type:	Doctoral Thesis
Press:	São Paulo.
Institution:	Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:	2008-06-10
Examining board members:	Dolores Ursula Mehnert; Glavur Rogerio Matte; Irma Nelly Gutierrez Rivera; Petra Sanchez Sanchez; Rene Peter Schneider
Advisor:	Dolores Ursula Mehnert
Abstract
The aim was to develop and evaluate a simplified methodology for detection of human enteric viruses in sewage sludge. The method was based on viral elution with protein solution, followed by ultracentrifugation. Several parameters were evaluated, including time and elution pH, clarifying and purifying conditions. The method was applied to the detection of adenoviruses, hepatitis A virus and noroviruses in sewage sludge samples collected for twelve months at two sewage treatment plants in Sao Paulo state. Raw sewage samples were also collected as a reference for viral presence. PCR, RT-PCR and Real-Time PCR revealed the presence of adenoviruses, including the enteric ones (species F) and hepatitis A virus found both in sewage and sludge. Noroviruses were not detected in any samples. Cell culture infectious viruses were not detected in the sludge subjected to chemical treatment (STP A), and statistical analyses revealed the efficiency of this treatment for virus inactivation. (AU)