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| Author(s): |
Priscila Larcher Longo
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-07-18 |
| Examining board members: |
Marcia Pinto Alves Mayer;
Antonio Olavo Cardoso Jorge;
Marilis do Valle Marques;
Adriana da Costa Ribeiro;
Elisabete Jose Vicente
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| Advisor: | Marcia Pinto Alves Mayer |
| Abstract | |
Aggregatibacter actinomycetemcomitans produces several virulence factors which induce periodontal destruction, but little is known about their regulation. The two components system ArcAB is a genetic transcription regulator that senses oxygen concentrations in the environment. This study aimed to construct an A. actinomycetemcomitans arcB deficient mutant and to compare phenotypic carachteristics and gene transcription with the wild type. The growth curves under microaerophilic and anaerobic conditions were similar. There were differences in abilities to adhere and invade epithelial cells, to form biofilm, to adhere to saliva-coated hydroxyapatite and in hydrophobicity between the strains. Gene transcription analysis by real time PCR showed differential expression of apaH, vppA, vapA and omp29. These data indicate that in A. actinomycetemcomitans, ArcB is able to detect redox conditions and is associated with colonization factors of the oral cavity, by regulating transcription of genes associated with virulence. (AU) | |