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| Author(s): |
Cleverson Busso
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-08-25 |
| Examining board members: |
Mário Henrique de Barros;
Ivan de Godoy Maia;
Francisco Gorgonio da Nobrega;
Carla Columbano de Oliveira;
Luis Eduardo Soares Netto
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| Advisor: | Mário Henrique de Barros; José Ribamar dos Santos Ferreira Júnior |
| Abstract | |
Coenzyme Q transports electrons from NADH and succinate dehydrogenase to the bc1 complex of the mitochondrial respiratory chain. Ten gene products are involved in coenzyme Q metabolism. This work focuses on the last gene to be described: COQ10. Considering the presence of coenzyme Q in the interior of cells containing this gene inactive, we analyzed the respiratory activity of coenzyme Q in coq10 mutants. By using Q cycle inhibitors, in the interior of the complex III of mitochondrial respiratory chain (antimycin A and mixotiazol), in coq10 lineage, it was noted the response to antimycin A, but not mixotiazol, suggesting the existence of semiquinones, which were also confirmed in tests of electron paramagnetic resonance. The possible transmission and distribution of coenzyme Q by Coq10p was studied by site-directed mutagenesis at critical positions of a tunnel present in the molecular model structure, as well as analysis of over-expression of the protein. This study confirms the central role of Coq10p in respiratory metabolism. (AU) | |