Full text
| |
| Author(s): |
Carolina Salcedo Rivillas
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-05-24 |
| Examining board members: |
Luis Carlos de Souza Ferreira;
José Gregorio Cabrera Gomez;
Irene da Silva Soares
|
| Advisor: | Luis Carlos de Souza Ferreira |
| Abstract | |
Flagellin of gram negatives bacteria actives the innate immune system resulting in adjuvants effects against coadministrated antigens. However, there is not information about immunological properties of flagellins produced by gram positive bacteria. This study aimed the evaluation of immunomodulator effects of Hag flagellin from B. subtilis. Vaccine formulations based in purified Hag and Ovalbumine protein (OVA) were administrated intranasal (i.n.) and subcutaneously (s.c.) in Balb/C and C57BL/6 mice and then the population of B and T lymphocytes were monitored for production of antibodies and citocines and/or surface markers expression. Results showed the immunomodulatory properties of Hag in both mice lineages regarding the induction of antigen specific antibodies when immunized with OVA and Hag by i.n. and s.c. via. Most promising results concerning the adjuvant effects observed on T cells activation were found in C57BL/6 mice. The results obtained open future perspectives for the study of the potential use of B. subtilis Hag flagellin as adjuvant in vaccines. (AU) | |
| FAPESP's process: | 10/04327-6 - Characterization of immunomodulatory and adjuvant effects of Bacillus subtilis flagellin |
| Grantee: | Carolina Salcedo Rivillas |
| Support Opportunities: | Scholarships in Brazil - Master |