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| Author(s): |
Luana de Cássia Umeda
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-08-20 |
| Examining board members: |
Dolores Ursula Mehnert;
Rita de Cássia Compagnoli Carmona;
José Luiz Negrão Mucci
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| Advisor: | Dolores Ursula Mehnert |
| Abstract | |
Human enteric viruses are common contaminants of raw sewage and subproducts of sewage treatment processes. In recent years, those viruses were recommend as new microbiological indicators in different matrices in Brazilian legislation and others countries, although some questions should be elucidated. At present, the aim was to evaluate and compare the efficiencies of standard and molecular virological methods for detection of human enteric viruses in sludge and reclaimed water samples. Rotavirus and adenovirus were experimentally spiked in the proposed matrices and virus recovery and detection limits established for each method and matrice. Viruses naturally presented in 25 samples of sludge and 25 samples of reclaimed water were assayed by all methods and results evaluated and compared for statistical significance. From all methods evaluated, ICC-PCR showed to be the most suitable for virus surveillance in sludge and reclaimed water. (AU) | |
| FAPESP's process: | 09/04460-0 - Classical and molecular methods applied to the detection and quantification of virus in sludge and treated sewage: determination of efficiency and detection limits |
| Grantee: | Luana de Cássia Umeda |
| Support Opportunities: | Scholarships in Brazil - Master |