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| Author(s): |
Julia Cortina Campopiano
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-06-11 |
| Examining board members: |
Joao Gustavo Pessini Amarante Mendes;
Silvia Beatriz Boscardin;
Marcela de Freitas Lopes
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| Advisor: | Joao Gustavo Pessini Amarante Mendes |
| Abstract | |
The interaction between innate and adaptative immunity occurs in several phases of the immune response. The Toll-like receptors (TLRs) have an important role in the activation of macrophages directly acting on the molecules secreted by these cells. Little is known about the role of these secreted molecules on the survival control of activated T lymphocytes (Activation-induced cell death - AICD). Therefore, the objective of the present work was to evaluate the effects of soluble factors produced by macrophages activated with several TLRs agonists, on the survival of T lymphocytes. First we sought the expression of TRLs on both bone marrow-derived and J774 macrophage cell line and we could see that both cells express all TLRs, except for TLR 11. The stimulation of both cells with TLRs agonists leads to the expression of NF-<font face=\"Symbol\">κB and the production of soluble factors that are able to protect DO11.10 T lymphocyte cell line from AICD, via down regulation of FasL partially mediated by PGE2. (AU) | |