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| Author(s): |
Susana Elaine Alves da Rosa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-09-30 |
| Examining board members: |
Maristela Martins de Camargo;
Eliana Faquim de Lima Mauro;
Aguinaldo Roberto Pinto
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| Advisor: | Maristela Martins de Camargo |
| Abstract | |
Common Variable Immunodeficiency (CVID) is characterized by hypogammaglobulinemia. Previously a CVID patient was identified with increased levels of Endoplasmic Reticulum (ER) stress due to dysregulation of the UPR. In the present study these analyses were performed in other patients and healthy donors. Maturation markers of B lymphocytes were also characterized in these individuals. Methods: Western-blot, RT-PCR, Q-PCR, Flow cytometry and culturing of ex vivo and immortalized B cells. Results: The analysis of 16 CVID patients and 9 healthy donors revealed three patients that present higher percentage of immature B cells in peripheral blood. Analysis of expression of BiP and XBP1 induced by LPS treatment of B lymphocytes from these patients revealed that one patient present increased levels of ER stress. (AU) | |
| FAPESP's process: | 09/06529-8 - Regulation of homeostasis of ER in B lymphocytes in common variable immunodeficiency |
| Grantee: | Susana Elaine Alves da Rosa |
| Support Opportunities: | Scholarships in Brazil - Master |