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| Author(s): |
Renato Brito Baleeiro
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-12-13 |
| Examining board members: |
Jose Alexandre Marzagao Barbuto;
Vera Lucia Garcia Calich;
Niels Olsen Saraiva Câmara;
Veronica Porto Carreiro de Vasconcellos Coelho;
Ramon Kaneno
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| Advisor: | Jose Alexandre Marzagao Barbuto |
| Abstract | |
Dendritic cells (DCs) are the main antigen presenting cells (APCs). It was proposed that DCs select the Ags that will be presented at their surfaces on class II based on the presence of a TLR-ligand on the cargo. However, the relevance of the coupling of the Ag to a TLR-ligand on class I was not yet addressed. Thus, the goal of this study was to investigate whether the coupling of particulate Ags to a TLR-agonist improve the cross-presentation (CP). We saw that the CP does not involve endosomal acidification and it relies on newly synthesized MHC-I molecules from the endoplasmic reticulum. Also, the presence of a TLR-ligand on the cargo neither contributed to the phagosome maturation nor to the endosomic route of CP. Furthermore, the maturation status of DCs was crucial for the induction of CD8+ T cells proliferation. Although the coupling of the Ag to the adjuvant has neither changed the expression of the markers related to maturation nor the presentation of the complex MHC-I/peptide at the DC surface, it affected its capacity of stimulating CD8+ T cells. (AU) | |