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| Author(s): |
Inaê Santiago do Nascimento
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-02-07 |
| Examining board members: |
Joao Gustavo Pessini Amarante Mendes;
Alexandre Salgado Basso;
Niels Olsen Saraiva Câmara
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| Advisor: | Joao Gustavo Pessini Amarante Mendes |
| Abstract | |
CD4+ T cells have the ability to differentiate into several subsets, such as Th1, with distinct abilities to fight infections. Once these cells exerted their effector functions, their elimination is necessary to restore the immune system homeostasis, which may occur by activation-induced cell death (AICD). Based on data previously obtained by our research group, this study aimed to investigate whether PGE2 protects in vitro differentiated CD4+ T cells, the same way that it protected DO11.10 hybridomas from AICD. To assess this, we used CD4+ T cells from total splenocytes or purified CD4+ T lymphocytes from C57Bl/ 6 wildtype mice, polarized to Th1 in the presence of anti-CD3, anti-CD28 and IL-12 for 4 days. We observed that the generated Th1 cells, in both conditions of purified CD4+ T cells or the ones from total splenocytes, were not protected from the process of AICD by PGE2, suggesting that this protection is not advantageous when these cells are already in advanced stages of their life cycle, thus avoiding the development of autoimmune diseases. (AU) | |
| FAPESP's process: | 09/13580-0 - Effect of Prostaglandin E2 on the expression of death receptors ligands and activation-induced cell death in CD4+ T Cells subsets. |
| Grantee: | Inaê Santiago Do Nascimento |
| Support Opportunities: | Scholarships in Brazil - Master |