Effect of the leucine overload in rat soleus muscle during gradual distraction osteogenesis.

Author(s)

Cãmila Valentim Deluca

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2007-09-26

Examining board members

Anselmo Sigari Moriscot; Patricia Chakur Brum; Chao Yun Irene Yan

• Advisor: Anselmo Sigari Moriscot

Abstract

The goal of the present study was to investigate the effect of leucine overload in the rat soleus muscle before and along DGO (Gradual Distraction Osteogenesis). Twenty five male Wistar rats were divided into 5 groups: control, sham-lengthening, leucine, DGO and DGO+leucine. The DGO was 2mm/day and the leucine overload (114mM) was administrated orally. Subsequently analyzed: histological cross-section, sarcomere serial number and cross-sectional area of the types fibers and connective tissue. The histological data showed that the DGO promoted intense injury and increase of connective tissue, which can be minimized by leucine. The sarcomere serial number increased by ~26% and leucine overload increased this gain to 33%. The fibers types showed a decrease of the cross-section area of the all type fibers with the DGO and increase of the type I fibers area with leucine overload. We conclude that leucine overload is a potential therapeutical device in DGO, seeking improvement of skeletal muscle longitudinal growth. (AU)