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| Author(s): |
Márcio José Ferreira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-04-09 |
| Examining board members: |
Estela Maris Andrade Forell Bevilacqua;
Silvia Daher;
Sergio Ferreira de Oliveira
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| Advisor: | Estela Maris Andrade Forell Bevilacqua |
| Abstract | |
Key cytokines such as IL-10 and IFN-<font face=\"symbol\">g, essential for immune response regulation, have also been found locally at maternal-placental interface during mouse pregnancy. Particularly, levels of IL-10 characterize an anti-inflammatory environment associated to the inhibition of T helper-1 lymphocytes (Th1) development and the proliferative stimulation of the B lymphocytes (humoral response). On the other hand, increases in IFN-<font face=\"symbol\">g profile prevent T helper-2 lymphocytes (Th2) activation leading to an inflammatory response that favors a Th1 response. The local production of these cytokines by NK uterine cells and T gd lymphocytes are relevant, but not exclusive. Thus, this study analyzed the potential contribution of the trophoblast in the maintenance of Th1/Th2 balance in the maternal-placental interface, represented, respectively by the expression of IL-10 and IFN-<font face=\"symbol\">g cytokines. The expression of the anti-inflammatory cytokine IL-10 and its receptor was evaluated in the presence of an inflammatory environment mimetized by IFN-<font face=\"symbol\">g addition to the culture medium. On contrary, the expression of the IFN-<font face=\"symbol\">g (and its receptor) was evaluated in the presence of IL-10 characterizing an anti-inflammatory condition. Mouse trophoblast cells were isolated from implantation sites at gestational day 7.5 and cultured in standard conditions. Gene and protein expression were determined by immunohistochemistry and RT-PCR. IL-10 and IFN-<font face=\"symbol\">g and their receptors were expressed in cultured trophoblast cells in the absence or presence of IFN-<font face=\"symbol\">g and IL-10, respectively. IFN-<font face=\"symbol\">g treatment increased IL-10R1 expression but do not alter IL-10 expression. On contrary, in the presence of IL-10 the IFN-<font face=\"symbol\">g expression increased significantly while the expression of its receptor decreased. These results suggest that a proinflammatory environment increases trophoblast responsiveness to IL-10 whereas an anti-inflammatory condition seems to reinforce the importance of IFN-<font face=\"symbol\">g expression at the maternal-placental interface, on the initial periods of gestation. (AU) | |