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| Author(s): |
Rodolfo Favaro Ribeiro
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-07-29 |
| Examining board members: |
Telma Maria Tenorio Zorn;
Paulo Alexandre Abrahamsohn;
Estela Maris Andrade Forell Bevilacqua;
Claudia Maria da Penha Oller do Nascimento;
Rita de Cassia Aleixo Tostes Passaglia
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| Advisor: | Telma Maria Tenorio Zorn |
| Abstract | |
By means of this study a new mouse model of pregnancy complicated by type 1 diabetes was established. Diabetes was induced in female Swiss mice by alloxan and the animals were mated after different periods of time. Diabetic females showed hyperglycemia, hypoinsulinemia, glycosuria, polyphagy, polydipsy and decreased body weight. Picrosirius histochemistry demonstrated increased fibrillar collagens in the decidua of the diabetic group. Immunohistochemical analysis showed increased collagens I and III, and decreased collagen V and proteoglycans biglycan and lumican. However, real time PCR analysis indicated that only collagen I mRNA was increased. Transmission electron microscopy revealed alterations in collagen fibrillogenesis. The myometrium showed alterations in the organization, cytoarchitecture and contractile system of the muscle layers, associated with decreased proliferation. These results contribute to explain the alterations in embryo development and the higher incidence of preterm labor in diabetic pregnancies. (AU) | |