The role of jaspamide in the actin cytoskeleton of melanoma cells: the relation between migration and invasion.

Author(s)

Michelle dos Santos Menezes

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-11-17

Examining board members

Glaucia Maria Machado Santelli; Mauricio da Silva Baptista; Fábio Siviero

• Advisor: Glaucia Maria Machado Santelli

Abstract

Many signaling ways are involved in metastasis and the cellular migration and invasion are important in this context. The role of microfilaments is essential in mesenchymal and amoeboid migration. In this context, the present work aimed to analyze the effects of the drug jaspamide and. after the determination of the IC50 concentrations for the HT144 and NGM cell lines, the effects of the treatment with jaspamide were studied. The wound assays indicated a decrease in the free area after the treatments, and the migration assays with transwell showed that, after the inoculation with the drug, the cells increased the process of migration. In the invasion assays with Boydens chamber, the treatment with jaspamide was effective only in NGM cells, when they are treated with 75 nM of the drug plus 30 <font face=\"Symbol\">mM of Y-27632. Regarding the migration process, the NGM cell line did not show movement when treated with the IC50 concentration and the IC50/2 concentration plus Y-27632, and the HT144 cell line increases this parameter when treated with jaspamide and 200 <font face=\"Symbol\">mM of NSC23766. (AU)

• FAPESP's process: 09/04474-1 - The jaspamide role in the dinamics of actin cytoskeleton of melanoma cells: relacion with migration and invasion
• Grantee: Michelle dos Santos Menezes
• Support Opportunities: Scholarships in Brazil - Master